The FGC (fast growing colony) line, a derivative of the LNCaP cell line shares all the main characteristics, including its androgen dependence, described for the original LNCaP cultures. A number of sublines originated from the FGC line which were characterized with respect to their response to steroid-depleted serum and to the synthetic androgen, R1881. After subcloning the FGC line a series of clones was isolated with distinct patterns of androgen-responsiveness. Among the sublines and clones studied, the FGC, FGC-JB and FGC clone-9 were androgen-dependent, whereas subline LNO, R and presumably also FGC clone-22 were androgen-independent. Distinct morphological differences were observed between the cells of the various sublines and between clone-9 and 22. The LNCaP cell line, its descending sublines and clonal derivatives provide a suitable in vitro model for studying different aspects of androgen-responsiveness of human prostate cancer.
LNCaP FGC：生長(zhǎng)很快。是LNCaP的一種衍生細(xì)胞系，含有LNCaP的所有主要特征形狀，包含雄性激素依賴性。很多源于FGC的細(xì)胞株能對(duì)固醇類缺乏的血清有反應(yīng)，和自我合成雄性激素，r1881. 克隆后，F(xiàn)GC細(xì)胞株表現(xiàn)出顯著的激素依賴行。在很多呀細(xì)胞株中和克隆中，F(xiàn)GC, FGC-JB and FGC clone-9是激素依賴性，但是亞細(xì)胞株 LNO, R and presumably also FGC 非激素依賴性。clone-22 和 clone-9形態(tài)學(xué)上有很大的差別。LNCaP細(xì)胞系及其他克隆為激素應(yīng)答的前列腺癌癥的研究提供了合適和可靠體外模型。
LNCaP FGC：生長(zhǎng)很快。是LNCaP的一種衍生細(xì)胞系，含有LNCaP的所有主要特征形狀，包含雄性激素依賴性。很多源于FGC的細(xì)胞株能對(duì)固醇類缺乏的血清有反應(yīng)，和自我合成雄性激素，r1881. 克隆后，F(xiàn)GC細(xì)胞株表現(xiàn)出顯著的激素依賴行。在很多呀細(xì)胞株中和克隆中，F(xiàn)GC, FGC-JB and FGC clone-9是激素依賴性，但是亞細(xì)胞株 LNO, R and presumably also FGC 非激素依賴性。clone-22 和 clone-9形態(tài)學(xué)上有很大的差別。LNCaP細(xì)胞系及其他克隆為激素應(yīng)答的前列腺癌癥的研究提供了合適和可靠體外模型。